Endophytic Fungi Isolated from Mangrove (Rhyzopora mucronata) and Its Antibacterial Activity on Staphylococcus aureus and Escherichia coli
A. A. Prihanto, M. Firdaus and R. Nurdiani
Dept. Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Veteran street, Malang, Indonesia
Abstract: Disc diffusion method was used to investigate the antibacterial activity of methanol extract of five endophytic fungi isolated from mangrove (Rhizopora mucronata) on Staphylococcus aureus ATCC 9144 andEscherichia coli ATCC 8739. Fungi were isolated from leaf, stem and root of R. mucronata. Potato dextrose agar and potato dextrose broth were used for fungi purification and fermentation. Fungal metabolites were extracted with methanol (1:1 v/v) for 24 h before vacuum evaporated to yield the crude extracts. Antimicrobial activity test was done according to the Kirby-Bauer test. The inhibition zone around each disc was considered to measure antibacterial activity of endophytic fungi. Only three endophytic fungi showed inhibition activity on S. aureus ATCC 9144 and two endophytic fungi showed inhibition on E. coli ATCC 8739. The results suggested that the extracts have a high potential for enteric diseases treatment caused by foodborne pathogen.
Key words: Antibacterial, endophytic fungi, mangrove, Rhyzopora mucronata.