Daily Archives: August 12, 2012

Phytochemical screening and Antibacterial activity of methanol extract of mangrove plant (Rhyzophora mucronata) from Porong River Estuary

12 August 2012

Rahmi Nurdiani, Muhamad Firdaus, Asep Awaludin Prihanto

 Dept. Fishery Product Technology, Faculty of Fisheries and Marine Science, Malang, Indonesia
asep_awa@ub.ac.id

Abstract

The aims of this study were to obtain phytochemical content and to examine antibacterial activity potency of methanol extract of Rhyzophora mucronata. The phytochemical screening was assayed by qualitative analysis and the antibacterial assay determined by disk diffusion method. The results of phytochemical analysis showed that all methanol extract of root, bark, leaf, fruit and flower of R. mucronata contained alkaloid, tannin, and flavonoid, in addition fruit contained steroid., respectively. The antibacterial activity in gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Escherichia coli) was showed by almost all extracts of root, bark, wood, flower and leaf of R. mucronata.

PERBANDINGAN AKTIVITAS ANTIBAKTERI Penicillium notatum ATCC 28089 DENGAN Penicillium sp. R1M YANG DIISOLASI DARI MANGROVE Sonneratia caseolaris

12 August 2012

Asep Awaludin Prihanto

Abstract

Mangrove Sonneratia caseolaris is a potential host for  endhopytic fungi that commonly produce strong dan unique antibiotic. The objective of the research was to compare the antibacterial activity of Penicillium notatum ATCC 28089 and endophytic fungus Penicillium sp. R1M isolated from Mangrove Sonneratia caseolaris. First step of the research was observation the growth curve of each isolate. Second step was isolation of their  metabolites to be used in the antibacterial activity assay against Staphylococcus aureus ATCC 9144 and Escherichia coli ATCC 8739. The results showed that both isolates indicated different growth curves. Penicillium sp. R1M experienced shorter adaptation phase, than P. notatum  ATCC 28089.  The secondary metabolites of  P. notatum ATCC 28089 was isolated on day 8, while those of Penicillium sp. R1M isolated on day 6. The result of antibacterial assay  confirmed  that the  extracts of mycelia Penicillium sp. R1M show inhibition diameter in the growth of S. aureus and E. coli  with 11.5 ± 0.6 mm and 10.6 ± 0.4, mm, respectively, while the medium extracts of  P. notatum ATCC 28089 were 9.3 ± 0.9 mm and 7.4 ± 0.1 mm, respectively. The  inhibitory zone diameter of  medium extracts. Penicillium sp. R1M against S. aureus and  E. coli were correspondingly 7.5 ± 0.1 mm and 7.1 ± 0.8 mm, while  the medium  extracts of P. notatum ATCC 28089 were 9.3 ± 0.6 mm and 7.4 ± 0.4 mm, respectively. Mycelial extracts of  Penicillium sp. R1M showed better antibacterial activity than those of  P. notatum ATCC 28089. On the other hand the medium extracts showed the opposite results.

Keywords: antibacterial, Penicillium sp. R1M, Sonneratia caseolaris

 

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Aktivitas Antimalaria (Plasmodium falciparum 3D7) dari Spon Laut Acanthella sp

12 August 2012

Faidzil Ikram, Asep Awaludin Prihanto, Choiriyatun Hanifah, Muhammad Iqbal

Abstract

Penelitian ini bertujuan untuk mendapatkan kandidat bioaktif anti multiresisten malaria dari bioaktif spon laut Acanthella sp.. Tahap penelitian diawali dengan mengisolasi senyawa dari Acanthella sp.. Selanjutnya dilakukan uji antimalaria secara invitro terhadap Plasmodium falciparum 3D7. Pengujian terhadap P. falciparum strain 3D7 menggunakan dosis konsentrasi bertingkat dengan kontrol positif digunakan chloroquin. Perhitungan penghambatan skizon dilakukan dengan pewarnaan darah dan selanjutnya dihitung jumlah eritrosit yang terinfeksi per 1000 eritrosit. IC50 dihitung menggunakan analisis probit. Hasil penelitian menunjukkan bahwa keempat fraksi aktif menunjukkan penghambatan pertumbuhan P. Falciparum dengan nilai IC50 yang berbeda-beda. Nilai terbaik dihasilkan oleh fraksi B dengan IC50 sebesar 0,013 ppm.